Going with the flow: how faecal bacteria spread in streams
Faecal bacteria – bacteria that are present in the digestive system of humans and animals – are known to contaminate waters. Little research has been conducted into the spread and distribution of faecal bacteria in rivers and, above all, into their input from the surrounding landscape. Researchers from the Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB) and from Scotland’s University of Aberdeen have developed an indicator-based model that can be used to assess the dynamics of faecal bacteria on the basis of hydrological processes in the landscape and the connectivity of streams – an important basis for managing the acute or sustained microbial contamination of waters.
To be able to understand the spread and fate of faecal bacteria in waters, scientists must also record the areas of origin, the quantity, and the flow paths of water in the landscape, and take into account inputs to the river network. Based on a study area in Scotland, Dörthe Tetzlaff from IGB, her doctoral student Aaron Neill and their colleagues from the University of Aberdeen (Chris Soulsby and Norval Strachan) explored the spread of labelled faecal indicator organisms in a catchment area. They then coupled the data to hydrometric values (quantitative determination of the hydrological regime) and stable isotope tracers. “Combining such data constitutes an innovative approach. Based on our findings, we developed a mathematical model on the spread of faecal bacteria, which can be transferred to other regions as well”, explained Chris Soulsby from the University of Aberdeen, the holistic concept of the study.
Exact prediction of faecal bacteria distribution in summer by the model:
Both the researchers’ field trials involving indicator bacteria and their mathematical model demonstrated that the degree of hydrological connectivity plays a crucial role in the distribution of faecal bacteria. Using this method, the team was able to quantify exactly when, and from where, bacteria enter the system. “In our study area, the concentrations of faecal bacteria in streams were highest in summer, which can also be expected in other regions. Summer is the most popular time for recreational use of rivers and intense rainfall events occur more frequently, flushing bacteria from surrounding areas into freshwater ecosystems. In summer, therefore, bacteria that do not originate from the immediate vicinity of the stream also enter the water, whereas in winter, bacteria stored in the riparian zone are mainly mobilised. The model we developed successfully captures the actual distribution of bacteria in summer,” stated Dörthe Tetzlaff, outlining the results of the study.
The researchers need to adapt the indicator-based model to improve its predictive accuracy for the winter months.. The researchers believe that the reason for this disparity could be the elevated mortality rate of bacteria at lower temperatures and frozen soil conditions, which the model not yet captures. “Optimising such a temporally and spatially dynamic mathematical model is a highly exciting, yet laborious task. There are so many unknowns that must be factored in,” concluded Dörthe Tetzlaff. However, the scientists are optimistic that the model, and its later versions, will one day represent the scientific basis for assessing the spread of faecal bacteria in the catchments of streams.
Further points of reference to the subject at IGB:
• Stress test for rivers: freely available scenario analysis tool, to investigate and visualize different stressors for European rivers. Dr. Markus Venohr
• Pharmaceuticals in surface waters and their impact on the physiology and reproduction of fish and amphibians. Prof. Dr. Werner Kloas
• Water as a potential transmission path (vector) of selected pathogenic diseases that have so far received little attention (e.g. Closterium deciphile, MRSA bacteria and the influenza A virus). Prof. Dr. Hans-Peter Grossart in the research network “Leibniz-Forschungsverbund INFECTIONS’21”.
Prof. Dr. Dörthe Tetzlaff: Dörthe Tetzlaff is Head of Department “Ecohydrology” at IGB and Full Professor at the Humboldt-Universität zu Berlin, Germany. Her international team investigates the entire water cycle in the landscape: in soil, groundwater, surface waters – but also in plants and the atmosphere. Dörthe Tetzlaff wants to learn more about the hydrology of catchments on different spatial and temporal scales. She studies the interactions of landscapes and watercourses to understand how the water regime influences the hydrochemistry and ecohydrology of surface waters.
About the Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB):
Work at IGB combines basic research with preventive research as a basis for the sustainable management of freshwaters. In the process, IGB explores the structure and function of aquatic ecosystems under near-natural conditions and under the effect of multiple stressors. Its key research activities include the long-term development of lakes, rivers and wetlands under rapidly changing global, regional and local environmental conditions, the development of coupled ecological and socio-economic models, the renaturation of ecosystems, and the biodiversity of aquatic habitats. Work is conducted in close cooperation with universities and research institutions from the Berlin-Brandenburg region as well as worldwide. IGB is a member of the Forschungsverbund Berlin e.V., an association of eight research institutes of natural sciences, life sciences and environmental sciences in Berlin. The institutes are members of the Leibniz Association. www.igb-berlin.de/en
Prof. Dr. Tetzlaff
Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Germany
Professor Christopher Soulsby
University of Aberdeen, Scotland
Aaron J. Neill; Doerthe Tetzlaff; Norval J. C. Strachan; Chris Soulsby: To what extent does hydrological connectivity control dynamics of faecal indicator organisms in streams? Initial hypothesis testing using a tracer-aided model. Journal of Hydrology. - 570(2019), S. 423-435