New potential therapeutic approach for HER2-positive breast cancer discovered
Resistance to HER2-targeted therapies can be a problem when treating patients with HER2-positive (HER2+) breast cancer. Therefore, the identification of new therapies for this patient group is important. Researchers at the Leibniz Research Centre for Working Environments and Human Factors in Dortmund (IfADo) have already shown that the enzyme EDI3 is associated with changes in the metabolism of cancer cells. Their most recent results reveal that inhibiting EDI3 may be a new therapeutic target in patients with therapy-resistant ER-HER2+ breast cancer.
Tumour cells alter their metabolism in order to maintain growth, thus ensuring their survival. Enzymes regulate metabolism and are therefore viable candidates for targeted cancer therapies. Researchers at IfADo have already identified one such enzyme – EDI3. The glycerophosphodiesterase EDI3, which cleaves glycerophosphocholine to choline and glycerol-3-phosphate, influences choline and phospholipid metabolism and has been linked to cancer-relevant functions in vitro. While the importance of choline metabolism in breast cancer has been studied, the role of EDI3 in this type of cancer has not yet been explored.
Targeting EDI3 as a therapeutic approach in ER-HER2+ breast cancer
In a recent study, the researchers showed that EDI3 expression is highest in ER-HER2+ breast tumours in humans and that both expression and enzymatic activity were highest in ER-HER2+ breast cancer cell lines. Silencing of HER2 as well as inhibition of HER2 signalling decreased EDI3 expression. Inhibition of EDI3, in turn, primarily reduced the viability of ER-HER2+ cells. Furthermore, inhibition of EDI3 in ER-HER2+ cells resistant to HER2-targeted therapy reduced cell viability in vitro and tumour growth in vivo in mice.
Based on these findings, the researchers conclude that EDI3 expression is upregulated in ER-HER2+ breast cancers compared to other subtypes. Furthermore, inhibiting EDI3 leads to a significant reduction in viability and tumour growth, especially in ER- HER2+ breast cancer cells that are resistant to conventional HER2-targeted therapies. Targeting EDI3 may therefore be a therapeutic approach to enhance the effect of standard therapies, or an alternative in the case of resistance to standard therapies.
Receptor status of the tumour
HER2 is the name of a growth factor receptor. Its task is to take up signals from outside the cell, conduct them into the cell interior, leading to the stimulation of cell division. If a patient has too much of the HER2 growth factor receptor, the tumor is referred to as HER2+ – a subtype of breast cancer. As a result of the many growth signals, the tumour can divide uncontrollably. Determining HER2 status is important since there are medications that specifically target HER2. Most HER2+ patients do respond successfully to such medications, but therapy resistance is a problem for some. Therefore, alternative treatments are needed for this tumor subtype.
In addition to HER2 signalling, the hormone oestrogen can also affect the growth of breast cancer cells. Oestrogen attaches to binding sites (hormone receptors) of the cell, which then turns on expression of genes that favours cell growth. To determine whether a tumour grows in a hormone-dependent manner, the proportion of cells and the amount of the corresponding hormone receptors is often examined. The result is expressed by the indication ER+ (oestrogen receptor positive) or ER- (oestrogen receptor negative).
Dr. Rosemarie Marchan
Phone: +49 231 1084-213
Keller, M., Rohlf, K., Glotzbach, A. et al. Inhibiting the glycerophosphodiesterase EDI3 in ER-HER2+ breast cancer cells resistant to HER2-targeted therapy reduces viability and tumour growth. J Exp Clin Cancer Res 42, 25 (2023). https://doi.org/10.1186/s13046-022-02578-w